PestPatrol runs on all 32-bit Windows platforms: Windows 95, Windows 98, Windows ME, Windows NT, Windows 2000 and Windows XP.

PestPatrol looks for many Unix-specific worms, trojans, and other problem code, and can scan a Unix drive that is configured with SAMBA. Under SAMBA, the drive looks just like a Win32 drive and would function as though it were an exposed network drive.

PestPatrol also scans for many pests specific to DOS or Windows 3.x, and can scan such drives if networked.